Amplicon sequencing on Illumina MiSeq of Aegilops geniculata: accessi... - SRA

SRX6356526: Amplicon sequencing on Illumina MiSeq of Aegilops geniculata: accession IG 48190
1 ILLUMINA (Illumina MiSeq) run: 49,408 spots, 29.7M bases, 19.5Mb downloads

Design: PCR primers of 20-26 bp were designed in exons encompassing intronic regions of about 400 bp for 48 low-copy nuclear loci, with an annealing temperature arounf 60 C. PCRs of all nuclear loci were carried simultaneously on 48.48 IFC plates following Fluidigm protocol. Amplicons were then pooled and barcoded by sample for paired-end (2x300 bp) Illumina MiSeq.

Submitted by: Institute of Biology

Study: Amplicon sequencing of low-copy nuclear loci in Aegilops species

PRJNA550182 • SRP211843 • All experiments • All runs

show Abstracthide Abstract

The dataset was produced to infer the evolutionary history of four diploid Aegilops spp. (Ae. tauschii, Ae. umbellulata, Ae. caudata and Ae. comosa) and of four of their derivative allopolyploid species (Ae. cylindrica, Ae. crassa, Ae. geniculata and Ae. triuncialis). The dataset includes 420 accessions of these eight species, 29 replicates and 26 outgroup species: all other extent diploid Aegilops spp., Triticum monococcum, Triticum urartu, Secale cereale, Hordeum vulgare and Taeniatherum caput-medusae.

Sample: Sample names are read as follows: "IndividualName_AccessionID_AccessArrayBarcode"

SAMN12110093 • SRS5019397 • All experiments • All runs

Organism: Aegilops geniculata

Library:

Name: TGTGAATCTC

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 49,408 spots, 29.7M bases, 19.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR9590599	49,408	29.7M	19.5Mb	2020-07-22

ID:: 8412274

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